Home
Class 12
BIOLOGY
One can ligate a foreign DNA at Bam H, s...

One can ligate a foreign DNA at Bam H, site of tetracycline resistance gene in the vector .........

Text Solution

AI Generated Solution

To solve the question regarding the ligation of foreign DNA at the BamHI site of a tetracycline resistance gene in a vector, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Context**: The question refers to a specific site (BamHI) in a vector that contains a gene for tetracycline resistance. Tetracycline is an antibiotic, and the presence of this gene allows bacteria to survive in the presence of the antibiotic. 2. **Identify the Vector**: A vector is a DNA molecule used to carry foreign genetic material into another cell. In this case, we need to identify a cloning vector that contains the BamHI site within the tetracycline resistance gene. ...
Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    MODERN PUBLICATION|Exercise Quick Memory Test ( C. Choose the correct alternative )|4 Videos
  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    MODERN PUBLICATION|Exercise REVISION EXERCISES (I. MULTIPLE CHOICE QUESTIONS (MCQs))|14 Videos
  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    MODERN PUBLICATION|Exercise Quick Memory Test ( A. Say True or False )|8 Videos
  • BIOTECHNOLOGY AND ITS APPLICATIONS

    MODERN PUBLICATION|Exercise CHAPTER PRACTICE TEST (SECTION - E)|2 Videos

Similar Questions

Explore conceptually related problems

If we ligate a foreign, DNA at the Barn HI site of tetracycline resistance gene in pBR322 the recombinant plasmid u ill:

In order to insert alien DNA at the site of tetracycline resistance gene in pBR322 the restriction endonuclease used can be :

Assertion : Recognition site should be perfectly single and responsive to commonly used restriction enzymes. Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

At which of the following restriction sites foreign DNA should be ligated to inactivate tetracycline gene in a vector like pBR 322

In pBR 322 amp^(R) and "tet"^(R) gene are present. When we ligate a foreign DNA at Pst-I site, then recombinant plasmids will lose the resistance to the

Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI