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Restriction endonucleases...

Restriction endonucleases

A

Are present in mammalian cells for degradation of DNA when the cell dies

B

Are used in genetic engineering for ligating two DNA molecules

C

A used in vitro DNA synthesis

D

Are synthesized by bacteria as part of their defence mechanism

Text Solution

Verified by Experts

The correct Answer is:
D
Are synthesized by bacteria as part of their defence mechanism
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Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?