Somatic hybridisation can be done by:

Options

A. Protoplast fusion
B. Cell culture
C. Haploid anther
D. Pollen culture

Explanation

**Step-by-Step Solution:**

1. **Understanding Somatic Hybridization:**
- Somatic hybridization refers to the process of fusing somatic cells, which are non-reproductive cells (2N), to create a new hybrid cell. This process does not involve the reproductive cells or gametes.

2. **Process of Somatic Hybridization:**
- The first step in somatic hybridization is the removal of the cell wall from the somatic cells. This is typically achieved using specific enzymes. For instance, if the cell wall is made of cellulose, the enzyme cellulase is used to digest the cell wall.

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