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Gel electrophoresis is a technique to se...

Gel electrophoresis is a technique to separate fragments of DNA from a mixture. Some of the events of electrophoresis are given below. Arrange the events in order.
(i) Cut out DNA bands (ii) Expose to UV (ii) Force DNA to move through gel (iv) Stain DNA with ethidium bromide.

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To arrange the events of gel electrophoresis in the correct order, we need to understand the sequence of operations involved in the process. Here’s the step-by-step solution: 1. **Force DNA to move through gel**: The first step in gel electrophoresis is applying an electric field to the gel, which causes the negatively charged DNA fragments to migrate towards the positive electrode. This movement separates the DNA fragments based on their size. 2. **Stain DNA with ethidium bromide (ETBR)**: After the DNA has been separated in the gel, the next step is to stain the DNA with ethidium bromide. This dye intercalates between the bases of the DNA and allows for visualization under UV light. 3. **Expose to UV**: Once the DNA is stained, the gel is exposed to UV light. The ethidium bromide emits an orange fluorescence when exposed to UV light, making the DNA bands visible. ...
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MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -REVISION EXERCISES (III. SHORT ANSWER TYPE -I QUESTIONS ( A. QUESTION FROM STATE BOARD EXAMINATIONS :))
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  3. Gel electrophoresis is a technique to separate fragments of DNA from a...

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  6. Figure representing the reactions associated with Polymer Chain Reacti...

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  7. BIOTECHNOLOGY & ITS PRINCIPLES

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  8. Write two significance of Bioreactors

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  9. What do you mean by term 'cloning' ?

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  10. How does restriction endonuclease work?

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  11. What are Molecular Scissors? Give one example.

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  12. Write a short note on bioreactor.

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  13. DOWNSTREAM PROCESSING

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  14. What is DNA probe? What are its uses?

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  15. The following photograph shows the result of a technique showing the s...

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  16. Different methods have been suggested to introduce alien DNA into host...

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  17. Write the methods to introduce alien DNA into host cells?

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  18. Draw a neat labelled diagram of plasmid pBR322.

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  19. What is the role of Ori for cloning vector?

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  20. Write the full name of ELISA.

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