Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.

Identify the restrcition site present in a structural gene other than antibiotic resistant gene in pB322.

Draw a schematic diagram of the E. coli cloning vector Pbr322 and mark the following in it : (a) ori (b) rop (c) ampicillin resistance gene (d) tetracycline resistance gene (e) restriction site BamHI (f) restriction site EcoRI

Which of the following restriction enzyme site is present in nucleotide sequence of ampicillin resistance gene in pBR322?

(a) Draw a schematic representation of the structure of a transcription unit and show the following in it : (i) Direction in which transcription occurs (ii) Polarity of the two strands involved (iii) Template strand (iv) Terminator gene (b) Mention the function of promoter gene in transcription.

Read the following and choose the correct statements . (A) First recombination DNA - Plasmid isolated from Salmonella and tagged with antibiotic resistance gene and cultured in E.coil. (B) Eco RI- It recognises a palindrome with 6 base pairs and 14 hydrogen bonds . (C) pBR 322 - 8 restriction sites for eigth types of restriction enzyme , two types of gene for antibiotic resistacne , one for gene replication of plasmid (Ori) . (D) Copy number - Ori gene that supports the production of more DNA copies .

Draw a neat diagram of female reproductive system and label the following: (a) Part where eggs are formed. (b) Site of fertilization. (c) Place of implantation. (d) Place for entry of sperms.

Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites, Ori, antibiotic resistance genes & ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.