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Read statements a-d. Which two of them h...

Read statements a-d. Which two of them have mistakes
(a) First transgenic buffalo Roise produced milk which was human alpha-lactalbumin enriched
(b) Restriction enzymes are used in isolation of DNA from other macromolecules
(c) Downstream processing is one of the steps of rDNA technology
(d) Disarmed pathogen vectors are also used in transfer of rDNA into the host

A

Statements (A) and (C)

B

Statements (A) and (B)

C

Statements (B) and (C)

D

Statements (C) and (D)

Text Solution

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The correct Answer is:
B

Statements (A) and (B)
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Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells with grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host.

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Each of the questions below consists of a question and two statements numbered I and II given below it. You have to decide whether the data provided in the statements are sufficient to answer the question. Read both the statements and give answer 1) if the data in statement I alone are sufficient to answer the question while the data in statement II alone are not sufficient to answer the question. 2) if the data in statement II alone are sufficient to answer the question while the data in statement I alone are not sufficient to answer the question. 3) if the data either in statement I alone or in statement II alone are sufficient to answer the question, 4) if the data even in both statements I and II together are not sufficient to answer the question. 5) if the data in both statements I and II together are necessary to answer the question. , A,B, C and D made their project presentation, one on each day, on four consecutive days but not necessarily in that order. On which day did ‘C’ make his presentation? I. The first presentation was made on 23rd, Tuesday and was followed by ‘D's presentation. II. 'A' did not make his presentation on 25th and one of them made his presentation, between A’s and B’s.

How many statements are correct? (a) Hind II always cut DNA molecules at particular point by recognizing a specific sequence of 6 bases. (b)After the restriction endonuclease action, single stranded portions which are overhanging stretches called sticky ends because they form nucleotide bonds with their complementary cut counter part. (c)The separated bands of DNA are cut out from the agarosegel and extracted from gel piece. This step is known as spooling. (d)Taq polymerase is used between annealing and extension of r-DNA technology.

Find out which among the following is true and which is false (i) Recombinant DNA technology has made it possible to engineer microbes, plants and animals such that they have novel capabilities. (ii) Genetically modified organisms have been created by using methods other than natural methods to transfer one or more genes from organism to another, generally using techniques such as recombinant DNA technology. (iii) GM plants have been useful in increasing crop yields, reduce post harvest losses and make crops more tolerant of stressess. (iv) Recombinant DNA technology process have made immense impact in the area of healthcare by enabling mass production of safe and more effective therapeutics. (v) since the recombinant therapeutics are identical to human proteins, they do not induced unwanted immunological responses.

MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -COMPETITION FILE ( OBJECTIVE TYPE QUESTION ( A. MULTIPLE CHOICE QUESTIONS ( MCQs):)
  1. A linear polymeric biomolecule with reducing and non-reducing ends is

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  2. If the length of a double helical DNA if 1.7 meters then the number of...

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  3. Read statements a-d. Which two of them have mistakes (a) First trans...

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  4. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  5. Gene amplification using primers can be done by :

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  6. Alec Jeffreys developed the DNA fingerprinting technique. The probe he...

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  7. Automated DNA sequencers, work on the peinciple of the method develope...

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  8. Tobacco plants resistant to a nematode have been developed by the in...

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  9. Biolistics (gene-gun) is suitable for

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  10. In genetic engineering, the antibiotics are used

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  11. In biotechnical processes cDNA is prepared from:

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  12. The figure below shows three steps (A,B,C) of Polymerase Chain Reactio...

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  13. It is normally a rare cancer but became a marker for AIDS/HIV patients

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  14. Cohen and Boyer isolated an antibiotic resistance gene, by cutting out...

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  15. Restriction enzyme Eco RI cuts the DNA between bases G and A only when...

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  16. Amplification of gene of interest by using DNA polymerase may go upto

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  17. In a genetic engineering experiment restriction enzymes can be used fo...

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  18. Which of the following is used to select genes of interest from a geno...

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  19. 'YAC'' refers to :

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  20. During amplification of gene using PCR Taq polymerase is used between:

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