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The figure below shows three steps (A,B,...

The figure below shows three steps (A,B,C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification togather with what it represents

A

B-denaturation at a temperature of about `98^(@)C` separating the two DNA strands.

B

A - denaturation at a temperature of about `50^(@)C`.

C

C-extension in the presence of heat stable DNA polymerase.

D

A - annealing with two sets of primers.

Text Solution

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The correct Answer is:
A

B-denaturation at a temperature of about `98^(@)C` separating the two DNA strands.
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MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -COMPETITION FILE ( OBJECTIVE TYPE QUESTION ( A. MULTIPLE CHOICE QUESTIONS ( MCQs):)
  1. In genetic engineering, the antibiotics are used

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  2. In biotechnical processes cDNA is prepared from:

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  3. The figure below shows three steps (A,B,C) of Polymerase Chain Reactio...

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  4. It is normally a rare cancer but became a marker for AIDS/HIV patients

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  5. Cohen and Boyer isolated an antibiotic resistance gene, by cutting out...

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  6. Restriction enzyme Eco RI cuts the DNA between bases G and A only when...

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  7. Amplification of gene of interest by using DNA polymerase may go upto

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  8. In a genetic engineering experiment restriction enzymes can be used fo...

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  9. Which of the following is used to select genes of interest from a geno...

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  10. 'YAC'' refers to :

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  11. During amplification of gene using PCR Taq polymerase is used between:

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  12. Which enzyme cuts DNA at specific sites?

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  13. The cutting of DNA at specific locations became possible with the disc...

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  14. Restriction endonucleases

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  15. A desirable change in genotype of an organism is obtained by

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  16. With respect to DNA fragmentation Statement A: Gel electrophoresis a...

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  17. Which of the following restriction enzymes produces blunt ends

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  18. The mechanism that causes a gene to move from one linkage group to ano...

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  19. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  20. Which of the following is not required for any of the techniques of DN...

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