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Restriction endonucleases...

Restriction endonucleases

A

Are synthesised by bacteria as part of their defense mechanism.

B

Are used for in vitro DNA synthesis.

C

Are used in genetic engineering for ligation of two DNA molecules.

D

Are present in mammalian cells for degradation of DNA when the cell dies.

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The correct Answer is:
A
Are synthesised by bacteria as part of their defense mechanism.
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Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?

MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -COMPETITION FILE ( OBJECTIVE TYPE QUESTION ( A. MULTIPLE CHOICE QUESTIONS ( MCQs):)
  1. 'YAC'' refers to :

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  2. During amplification of gene using PCR Taq polymerase is used between:

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  3. Which enzyme cuts DNA at specific sites?

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  4. The cutting of DNA at specific locations became possible with the disc...

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  5. Restriction endonucleases

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  6. A desirable change in genotype of an organism is obtained by

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  7. With respect to DNA fragmentation Statement A: Gel electrophoresis a...

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  8. Which of the following restriction enzymes produces blunt ends

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  9. The mechanism that causes a gene to move from one linkage group to ano...

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  10. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  11. Which of the following is not required for any of the techniques of DN...

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  12. Which of the following is a restriction endonuclease ?

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  13. Which of the following is not a feature of the plasmids

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  14. The taq polymerase enzyme is obtained from

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  15. Which of the following r-RNAs acts as structural RNA as well as ribozm...

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  16. Stirred-tank bioreactors have been designed for :

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  17. The DNA fragments separated on an agarose gel can be visualised after ...

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  18. Which of the following are not polymeric

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  19. Which of the following is commonly used as a vector for introducing a ...

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  20. Following statements describe the characteristics of the enzyme Restri...

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