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Gel electrophoresis is used for...

Gel electrophoresis is used for

A

Construction of recombinant DNA by joining with cloning vectors

B

Isolation of DNA molecules

C

Cutting of DNA into fragments

D

Separation of DNA fragments to their size

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Assertion (A) : Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion. Reason (R) : Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzyme.

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis.

Gel electrophoresis is employed in . I. Separation of DNA fragmetns . II. Selection of DNA fragments . III. Vissualizing DNA IV. Check the progression of restriction enzyme digestion.

The most commonly used matrix in gel electrophoresis is agarose, which is a natural polymer extracted from :-

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