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Write any four ways used to introduce a ...

Write any four ways used to introduce a desired DNA segment into a bacterial cell in recombinant technology experiments.

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(i) With the help of vectors .
(ii) Vectorless (Direct) gene transfer :
(a) Electrporation `" "` (b) Microinjection .
(c) Chemical mutated gene transfer `" "`(d) Gene gun or bioplastics.
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MODERN PUBLICATION-BIOTECHNOLOGY AND ITS APPLICATIONS-REVISION EXERCIES ( SHORT ANSWER TYPE - I QUESTION )
  1. Match the followig :

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  2. Insulin getting assembled into a mature form was the major challenge i...

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  3. Why does the Bt toxin not kill the bacterium that produces it but kill...

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  4. What is gene therapy? Name the first clinical case where it was used.

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  5. What are cry protein ? and organisms?

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  6. One of the application of biotechnology is to get pest resistant plan...

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  7. Mention any three advantages of GM organisms .

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  8. What is the characteristic feature of human insulin ?

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  9. List any four advantages of genetically modified crop plants over thei...

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  10. Name the type of bioreactor shown. Write the purpose for which it use...

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  11. Why is the introduction of genetically engineered lymphocytes into an...

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  12. In which technique do we use Taq polymerase and why ?

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  13. Expand the following : (a) AIDS (b) ELISA

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  14. What are Satellite DNA in a genome? Explain their role in DNA fingerpr...

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  15. Describe the gene therapy procedure for an ADA deficient patient

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  16. Expand the following and mention one application of each : (i) PCR (ii...

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  17. Name the source of the DNA polymerase used in PCR technique. Mention w...

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  18. Write any four ways used to introduce a desired DNA segment into a bac...

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  19. What is Bt toxin ? Name an organism that produces it ? How has man ex...

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  20. Name the bacterium responsible for the large holes seen in "Swiss Chee...

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