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Would you like lo choose an exonuclease ...

Would you like lo choose an exonuclease enzyme while producing a recombinant DNA molecule?

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No. this is because exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
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OSWAAL PUBLICATION-BIOTECHNOLOGY PRINCIPLES AND PROCESSES -TOPIC -2 PROCESS OF RECOMBINANT DNA TECHNOLOGY (MULTIPLE CHOICE QUESTIONS )
  1. Would you like lo choose an exonuclease enzyme while producing a recom...

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  2. Rising of dough is due to :

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  3. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

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  4. The transfer of genetic material from one bacterium to another through...

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  5. Which of the given statement is correct in the context of observing DN...

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  6. 'Restriction' in Restriction enzyme refers to :

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  7. A recombinant DNA molecule can be produced in the absence of the follo...

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  8. In agarose gel electrophoresis, DNA molecules are separated on the bas...

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  9. The most import.mt feature in a plasmid to be used as a vector is :

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  10. While isolating DNA from bacteria, which of the following enzymes is n...

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  11. Which of the following has popularised the PCR (polymerase chain react...

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  12. An antibiotic resistance gene in a vector usually helps in the selecti...

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  13. Significance of 'heat shock' method in bacterial transformation is to ...

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  14. The role of DNA ligase in the construction of a recombinant DNA molecu...

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  15. Which of the following bacteria is not a source of restriction endonuc...

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  16. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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  17. A bacterial cell was transformed with a recombinant DNA that was gener...

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  18. Which of the following should be chosen for best yield if one were to ...

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  19. Who among the following was awarded the Nobel Prize for the developmen...

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  20. Which of the following statements does not hold true for restriction e...

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