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Name the five key tools for accomplishin...

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Text Solution

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(i) Restriction endonucleases : For cutting the desired DNA at desired places
(ii) Gel electrophoresis: For separating the desired DNA fragments
(iii) Ligase enzyme: For creating recombinant DNA molecule. (iv) DNA delivery system : Like electroporation, microinjection, gene gun method.
(v) Competant host (usually bacteria / yeast) : To take up recombinant DNA.
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