Home
Class 12
BIOLOGY
. Explain in brief the separation and is...

. Explain in brief the separation and isolation of DNA fragments.

Text Solution

Verified by Experts

Seperations and isolation of DNA fragments.
(i) DNA cut by REN, fragments separated by gel electrophoresis.
(ii) Negatively chargeds DNA fragments are forced to move towards anode under electric field through medium/matrix.
(iii) Matrix used is agarose gel (PAGE/AGE).
(iv) DNA fragments are sepearated according to their size through sieving effect.
(v) Separated fragments are visualised in ethidium bromide, exposed to UV radiation.
(vi) Separated bands of DNA are cutout from agarose gel-Elution.
Doubtnut Promotions Banner Mobile Dark
|

Topper's Solved these Questions

  • SOLVED PAPER (II PUC MARTCH -2016)

    OSWAAL PUBLICATION|Exercise PART-D (SECTION - I)|7 Videos

  • SOLVED PAPER (II PUC MARTCH -2016)

    OSWAAL PUBLICATION|Exercise PART-D (SECTION - II)|8 Videos

  • SOLVED PAPER (II PUC MARTCH -2016)

    OSWAAL PUBLICATION|Exercise PART-B|8 Videos

  • SOLVED PAPER II PUC TOPPER'S ANSWER MARCH - 2017

    OSWAAL PUBLICATION|Exercise PART D (SECTION - II)|5 Videos

  • SOLVED PAPER (II PUC TOPPER'S ANSWER MARCH -2016)

    OSWAAL PUBLICATION|Exercise PART-D (SECTION -II) (Anwer any three of the following questions in about 200 to 250 words each wherever applicable)|5 Videos

Similar Questions

Explore conceptually related problems

DNA fragments are

Name the technique involved in separation and isolation of DNA fragment. Which dye is used to stain gel to make the DNA visible under UV light.

(a) Describe an activity along with a labelled diagram the phenomenon of dispersion through a prism. (b) Explain in brief the formation of rainbow with the help of figure.

Explain why carbon forms compounds mainly by covalent bonds. Explain in brief two main reasons for carbon forming a large number of compounds. Why does carbon form strong bonds with most other elements.

What is speciation? Explain in brief the role of natural selection and genetic drift in this process.

Name the enzyme that joins DNA fragments.

Explain in brief the role of animal husbandry in human welfare.

Explain in brief the role of animal husbandry in human welfare.

Write a brief note on predation.

Explain in brief the reason for each of the following : (i) Advanced sun-rise (ii) Delayed sun-set (iii) Twinkling of stars

Home
Profile