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(A) : Lysine (PI = 9.6) and glycine (PI ...

(A) : Lysine (PI = 9.6) and glycine (PI = 5.97) could be separated by electrophoresis
(R ) : In the electrophoresis process if the pH of the solution is maintained either at 9.6 or 5.97, one will migrate towards electric pole while other wouldn't.

A

Both (A) and (R ) are true and (R ) is the correct explanation of (A)

B

Both (A) and (R ) are true and (R ) is not the correct explanation of (A)

C

(A) is true but (R ) is false

D

(A) is false but (R ) is true

Text Solution

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The correct Answer is:
A
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(A) : An aqueous solution of glycine of pH = 7 is not electrically neutral. (R ) : The isoelectric point of glycine, PI is 6.0.

alpha - amino acids are high melting crystalline solids because of the zwitterion structure. They are moderately soluble in water. In acidic medium, alpha - amino acids exist as cations (I) and thus migrate towards cathode under the influence of an electric field. On the other hand, in alkaline medium, alpha - amino acids exist as anions (III) and thus migrate towards anode under the influence of an electric field. However, at some intermediate value of p^(H) , the concentration of the cationic form (I) and anionic form (III) will become equal and consequently the alpha - amino acid will exist primarily as the neutral dipolar ion (II). At this p^(H) , there would be no net migration of the amino acid in an electric field. This p^(H) at which there is no net migration of the amino acid under the influence of an applied electric field is called isoelectric point (pI). Each amino acid has a characteristic isoelectric point. The pH of an amino acid that does not have an ionisable side chain such as alanine isd average of pK_(a) values of the carboxyl group and the protonated amino group. H_(3)overset(+)(N)-overset("R")overset("|")("C")H-COOH overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr H_(3)overset(+)(N)-underset("Zwitterion (II)")(overset("R")overset("|")("C")H)-COO^(-)overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr underset((III))(overset(+)(N)-overset("R")overset("|")("C")H)-COO^(-) Further, the alpha - carbon of all the amino acids (except glycine) is chiral (asymmetric) and hence amino acids can exist in teo stereoisomeric forms i.e., D and L. However, all the nautrally occurring amino acids belong to the L - series. underset("L(-)Glyceraldehyde")(HO-overset("CHO ")overset("| ")underset(" "CH_(2)OH)underset("| ")("C ")-H)" " underset("L-Amino acid")(H_(2)N-overset("COOH")overset("| ")underset("R ")underset("| ")("C ")-H) Which of structural formula of lysine (NH_(2)-overset("COOH")overset("| ")("C ")H-CH_(2)-CH_(2)-CH_(2)-CH_(2)NH_(2)) at pH 13 ?

alpha - amino acids are high melting crystalline solids because of the zwitterion structure. They are moderately soluble in water. In acidic medium, alpha - amino acids exist as cations (I) and thus migrate towards cathode under the influence of an electric field. On the other hand, in alkaline medium, alpha - amino acids exist as anions (III) and thus migrate towards anode under the influence of an electric field. However, at some intermediate value of p^(H) , the concentration of the cationic form (I) and anionic form (III) will become equal and consequently the alpha - amino acid will exist primarily as the neutral dipolar ion (II). At this p^(H) , there would be no net migration of the amino acid in an electric field. This p^(H) at which there is no net migration of the amino acid under the influence of an applied electric field is called isoelectric point (pI). Each amino acid has a characteristic isoelectric point. The pH of an amino acid that does not have an ionisable side chain such as alanine isd average of pK_(a) values of the carboxyl group and the protonated amino group. H_(3)overset(+)(N)-overset("R")overset("|")("C")H-COOH overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr H_(3)overset(+)(N)-underset("Zwitterion (II)")(overset("R")overset("|")("C")H)-COO^(-)overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr underset((III))(overset(+)(N)-overset("R")overset("|")("C")H)-COO^(-) Further, the alpha - carbon of all the amino acids (except glycine) is chiral (asymmetric) and hence amino acids can exist in teo stereoisomeric forms i.e., D and L. However, all the nautrally occurring amino acids belong to the L - series. underset("L(-)Glyceraldehyde")(HO-overset("CHO ")overset("| ")underset(" "CH_(2)OH)underset("| ")("C ")-H)" " underset("L-Amino acid")(H_(2)N-overset("COOH")overset("| ")underset("R ")underset("| ")("C ")-H) In alanine, carboxyl group ionises at pK_(a1)=2.34 and ammonium ion at pK_(a2)=9.69 . The isoelectric point of the amino acid is at p^(H) .

alpha - amino acids are high melting crystalline solids because of the zwitterion structure. They are moderately soluble in water. In acidic medium, alpha - amino acids exist as cations (I) and thus migrate towards cathode under the influence of an electric field. On the other hand, in alkaline medium, alpha - amino acids exist as anions (III) and thus migrate towards anode under the influence of an electric field. However, at some intermediate value of p^(H) , the concentration of the cationic form (I) and anionic form (III) will become equal and consequently the alpha - amino acid will exist primarily as the neutral dipolar ion (II). At this p^(H) , there would be no net migration of the amino acid in an electric field. This p^(H) at which there is no net migration of the amino acid under the influence of an applied electric field is called isoelectric point (pI). Each amino acid has a characteristic isoelectric point. The pH of an amino acid that does not have an ionisable side chain such as alanine isd average of pK_(a) values of the carboxyl group and the protonated amino group. H_(3)overset(+)(N)-overset("R")overset("|")("C")H-COOH overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr H_(3)overset(+)(N)-underset("Zwitterion (II)")(overset("R")overset("|")("C")H)-COO^(-)overset(" "OH^(-)" ")underset(" "H^(+)" ")hArr underset((III))(overset(+)(N)-overset("R")overset("|")("C")H)-COO^(-) Further, the alpha - carbon of all the amino acids (except glycine) is chiral (asymmetric) and hence amino acids can exist in teo stereoisomeric forms i.e., D and L. However, all the nautrally occurring amino acids belong to the L - series. underset("L(-)Glyceraldehyde")(HO-overset("CHO ")overset("| ")underset(" "CH_(2)OH)underset("| ")("C ")-H)" " underset("L-Amino acid")(H_(2)N-overset("COOH")overset("| ")underset("R ")underset("| ")("C ")-H) N - terminus of the peptide structure is on the

Which of the following is the major solute species in a solution of lysine at pH = 10.5(pI = 9.6) ?

You have a mixture of three amino acids : E(pI = 3.2), Y(pI = 5.7) & K(pI = 9.7). Under electro - phoresis at pH = 7.7, in which direction will each component of the mixture move ?

Two short magnets have their magnetic moments 1.2 Am^(2) and 1.0 Am^(2) .They are placed on a horizontal table parallel to each other at a distance of 20 cm between their centres , such that their north poles pointing towards geographic south .They have common magnetic euatorial line . Horizontal componet of earth 's field is 3.6xx10 ^(-5) T. Then , the resultant horizontal magnetic induction at mid point of the line joining their centres is ( (mu0)/(4pi)=10^(-7)N//m)

The following questions consist of two statements, one labelled as 'Statement I' and the other 'Statement II'. You have to examine these two statements carefully and decide if the Statement I and the Statement II, are individually true and if so, whether the Statement II is the correct explanation for the given Statement I. Select your answer to these items using the codes given below and then select the correct option. Statement 1 : sin^(-1)(sin5) gt x^(2)-4x has many solutions lying in the interval (2-sqrt(9-2pi), 2+sqrt(9-2pi)) Statement 2 : Sin^(-1)(Sin5)=5-2pi

The following aqueous solutions given in column containing 1:1 mole ratios of the respective pairs of substances. Assume all concentrations are I M. {:("List-I", " " "List-II"),((A) NOH+NH_(4)OH,P."A buffer with a pH less than 7"),((B)NaOH+HCI,Q."A buffer with a pH greater than 7"),((C)HCN(P_(ka)=9)+KCN,R."The solution with a pH of 7"),((D) C_(6)H_(5)NH_(2)(P_(ka)=9.5)+C_(6)H_(5)NH_(3)""^(+)Cl^(-),S."The solution with the highest pH "):}

{:(HOOC-CH_(2)-underset(NH_(2))underset("| ")("C ")H-COOH,underset((-COOH))(pK_(a_(1))),underset((-NH_(2)))(pK_(a_(2))),underset(("Acid or base of "-R))(pK_(a_(3)))),(" Aspartic acid"," 2.1"," 9.8"," 3.9"),(HOOC-underset(NH_(2))underset("| ")("C ")H-(CH_(2))_(4)-NH_(2),underset((-COOH))(pK_(a_(1))),underset((-NH_(2)))(pK_(a_(2))),underset(("Acid or base of "-R))(pK_(a_(3)))),(" Lysine"," 2.2"," 9.0"," 10.5"),(HOOC-underset(NH_(2))underset("| ")("C ")H-CH-OH,,,),(" Threonine"," 2.6"," 10.4",),(HOOC-underset(NH_(2))underset("| ")("C ")H-CH_(2)CH_(2)CONH_(2),,,),(" Glutamine"," 2.2"," 9.1",):} The aqueous solution of lysine is neutral at

AAKASH SERIES-PROTEINS AND NUCLEIC ACIDS -PRACTICE SHEET - 4
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  2. Anti bodies are ………..

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  3. Proteins overset(" x ")underset(H(2)O)rarr Aminoacids 'x' is an enz...

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  4. Composition of DNA is …………

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  5. Insulin is an example of

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  6. In DNA strand at 136^(th) position is the base thymine, then the base ...

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  7. Which of the following has an aromatic ring ?

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  8. At p^(H)=4 Glycine exist as

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  9. Which of the following element is present in insulin ?

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  10. Iodine value is related to in our system is

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  11. Acrolin test is negative for

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  12. The waxes are long chain compounds of fatty acids and they does not be...

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  13. Which of the following/s is/are not deficiency disease of vitamine - E

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  14. Kwashiorkar is caused by the deficiency of

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  15. The number of Amino acids in the two protomers of insulin are

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  16. Walls of blood vessels, ligaments are called …………

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  17. Which of the following is true statement

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  18. Proteins can also be classified based on their shapes and solubility i...

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  19. Proteins can also be classified based on their shapes and solubility i...

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  20. Proteins can also be classified based on their shapes and solubility i...

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  21. The deficiency of enzyme in living system can cause many diseases. For...

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