Gene of interest was cloned at site Sal I in Pbr322. The recombinant plasmid will exhibit susceptibilty to

Options

A. Tetracycline
B. Tetracycline and Ampicilin
C. Ampilicin only
D. Broad spectrum of antibodies

Answer

To solve the question regarding the susceptibility of the recombinant plasmid containing the gene of interest cloned at the Sal I site in the PBR322 vector, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the PBR322 Vector**:
- PBR322 is a plasmid that contains genes for antibiotic resistance, specifically for ampicillin and tetracycline.

2. **Insertion of Gene of Interest**:
- The gene of interest is cloned into the Sal I site of the PBR322 plasmid. This insertion disrupts the tetracycline resistance gene.

3. **Effect of Gene Insertion**:
- When the gene of interest is inserted into the tetracycline resistance gene, it leads to the inactivation of this gene. As a result, the recombinant plasmid will no longer confer resistance to tetracycline.

4. **Antibiotic Susceptibility**:
- Since the tetracycline resistance gene is disrupted, the recombinant plasmid will exhibit susceptibility to tetracycline. This means that if tetracycline is present in the growth medium, the bacteria containing this recombinant plasmid will not survive.

5. **Resistance to Other Antibiotics**:
- The plasmid still retains its resistance to ampicillin, meaning it will not be susceptible to ampicillin.

6. **Conclusion**:
- Therefore, the recombinant plasmid will exhibit susceptibility to tetracycline.

### Final Answer:
The recombinant plasmid will exhibit susceptibility to **Tetracycline**.

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