State the functions of the following in the cloning vector pBR322 :
(i) Ori,
(ii) rop, and
(iii) Hind III sites.

Explanation

To answer the question regarding the functions of specific components in the cloning vector pBR322, we will discuss each component one by one:

### Step-by-Step Solution:

1. **Function of Ori (Origin of Replication)**:
- **Definition**: Ori stands for the "Origin of Replication".
- **Function**: It is a specific sequence of nucleotides where the replication of the plasmid begins. This site is crucial because it allows the plasmid to be copied within the host cell. The Ori serves as the initiation site for DNA replication, ensuring that the plasmid can be replicated and passed on to daughter cells during cell division.

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