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Rearrrange the following in the correct ...

Rearrrange the following in the correct sequence to accomplish an important biotechnological reaction:
( a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligo - nucleqtides
( c) Enzyme DNA - polymerase
( d) Complementary region of DNA
( e) Gernomic DNA template
(f) Nuceltides provided
( g)Primes
(h) Thermostable DNA -polymerase (from Thermus aquaticus )
( i) Denaturation of ds- DNA

Text Solution

AI Generated Solution

To rearrange the steps involved in the Polymerase Chain Reaction (PCR) for the synthesis of DNA, we can follow this logical sequence: 1. **(b) Chemically synthesized oligonucleotides** - These are the primers that will initiate the synthesis of the DNA. 2. **(f) Nucleotides provided** - The necessary building blocks (nucleotides) for DNA synthesis must be available. 3. **(h) Thermostable DNA-polymerase (from Thermus aquaticus)** - This enzyme is crucial for synthesizing new DNA strands at high temperatures. 4. **(i) Denaturation of ds-DNA** - The double-stranded DNA (ds-DNA) is heated to separate it into two single strands. 5. **(g) Primers** - The synthesized oligonucleotides (primers) bind to the complementary regions of the single-stranded DNA. 6. **(e) Genomic DNA template** - The template DNA serves as the original strand from which copies will be made. ...
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