Identify and illustration in the following

Explanation

(i) A-AATTC (Recognition Site of the restriction endonuclease)
(ii) B-Pru II (Origin of replication)
(b) Palindromic nucleotide sequence is depicted from A & C reads same on the strands when orientations of reading is kept the same.
A - Coding strand
B - Template strand
C - PCR strand for polymerase chain reaction. PCR is used to amplify DNA segments to a large number with in a short span of time.