What would happen if you forget to add cytokinin to the culture medium ?

Options

A. Callus will not develop shoot buds
B. Callus will not develop root buds
C. Callus will stop differentiating
D. Both (a) and (b)

Answer

### Step-by-Step Solution:

1. **Understanding Cytokinins**: Cytokinins are important plant hormones that play a crucial role in cell division and differentiation in plants. They are especially significant in tissue culture, where they help in the growth and development of plant tissues.

2. **Role of Cytokinins in Callus Culture**: In plant tissue culture, particularly in callus culture, the addition of cytokinins is essential for the development of shoot buds from the callus. Callus is an undifferentiated mass of cells that can develop into various plant structures.

3. **Effect of Omitting Cytokinins**: If cytokinin is not added to the culture medium, the callus will not be able to differentiate into shoot buds. This means that the process of shoot formation will be inhibited.

4. **Analyzing the Options**:
- **Option A**: Callus will not develop shoot buds. (This is true because cytokinin is necessary for shoot development.)
- **Option B**: Callus will not develop root buds. (While cytokinin is important for shoot development, auxins are primarily responsible for root development, so this option is not necessarily true.)
- **Option C**: Callus will stop differentiating. (This is not entirely accurate; the callus may still grow but will not differentiate into shoots without cytokinin.)
- **Option D**: Both A and B. (Since B is not true, this option is also incorrect.)

5. **Conclusion**: The correct answer is **Option A**: Callus will not develop shoot buds.