Callase enzyme which dissolved callose of pollen tetrads to separate four pollens is provided by

Options

A. pollens
B. tapetum
C. middle layers
D. endothecium

Explanation

To answer the question about which structure provides the callase enzyme that dissolves callose in pollen tetrads, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Pollen Tetrads**:
- Pollen tetrads are groups of four pollen grains that are formed from a single microspore mother cell through meiosis. These grains are initially held together by a substance called callose.

2. **Identifying the Role of Callase**:
- Callase is an enzyme that breaks down callose, allowing the individual pollen grains to separate from one another. This is a crucial step in the development of functional pollen grains.

3. **Analyzing the Options**:
- **Pollen**: While pollen grains are the end product, they do not produce callase.
- **Tapetum**: The tapetum is a layer of cells within the anther that provides nourishment to developing pollen grains and is responsible for synthesizing callase.
- **Middle Layers**: These layers provide some nourishment but do not synthesize callase.
- **Anther**: The anther is the part of the stamen that contains the pollen sacs but does not directly produce callase.

4. **Conclusion**:
- The tapetum is the structure that synthesizes the callase enzyme, which dissolves the callose in pollen tetrads, allowing the pollen grains to separate. Therefore, the correct answer is **tapetum**.

### Final Answer:
The callase enzyme which dissolves callose of pollen tetrads to separate four pollens is provided by **tapetum**.

---