Which of the following shows the correct sequence of steps of plants tissue culture ?

Options

A. Sterilisation `rarr` Hardening `rarr` Selection of explant `rarr` Inoculation `rarr` Regeneration `rarr` Plantlet transfer
B. Selection of explant `rarr` Inoculation `rarr` Regeneration `rarr` Sterilisation `rarr` Hardening `rarr` Plantlet transfer
C. Selection of explant `rarr` Sterilisation `rarr` Inoculation `rarr` Regeneration `rarr` Hardening `rarr` Plantlet transfer
D. Hardening `rarr` Sterilisation `rarr` Selection of explant `rarr` Inoculation `rarr` Regeneration `rarr` Plantlet transfer

Answer

**Step-by-Step Solution:**

1. **Selection of Explant**: The first step in plant tissue culture is to select the appropriate explant. This could be any part of the plant, such as leaves, stems, or roots, depending on the requirements of the culture.

2. **Sterilization**: After selecting the explant, the next step is to sterilize it. This is crucial to eliminate any potential contaminants, such as bacteria or fungi, that could interfere with the growth of the new plant.

3. **Inoculation**: Once the explant is sterilized, it is then inoculated into a nutrient medium. This medium provides the necessary nutrients for the explant to grow and develop.

4. **Regeneration**: The explant, due to its totipotency, begins to regenerate. This means it has the ability to develop into a whole new plant.

5. **Hardening**: After regeneration, the plant undergoes a hardening process. This step is important to prepare the plantlet for transfer to an external environment.

6. **Transfer to Garden**: Finally, once the plantlet has matured, it is transferred to the garden or a suitable growing environment where it can continue to grow independently.

**Correct Sequence**:
Selection of Explant → Sterilization → Inoculation → Regeneration → Hardening → Transfer to Garden

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