The enzymes required to obtain protoplast from a plant cell are

Options

A. cellulase
B. chitinase
C. pectinase
D. both (a) and (c ).

Explanation

To answer the question regarding the enzymes required to obtain protoplasts from plant cells, we can follow these steps:

### Step-by-Step Solution

1. **Understanding Protoplasts**:
- Protoplasts are plant cells that have had their cell walls removed. This allows for various applications in plant biotechnology, such as genetic engineering and somatic hybridization.

2. **Identifying the Components of the Cell Wall**:
- The cell wall of plant cells is primarily composed of polysaccharides, which include cellulose and pectin.

3. **Enzymatic Digestion of the Cell Wall**:
- To isolate protoplasts, the cell wall must be digested. This process requires specific enzymes that can break down the components of the cell wall.

4. **Enzymes Required**:
- The key enzymes involved in the digestion of the cell wall are:
- **Cellulase**: This enzyme breaks down cellulose, which is a major component of the cell wall.
- **Pectinase**: This enzyme breaks down pectin, another important component of the cell wall.
- **Chitinase**: This enzyme is not typically involved in the digestion of plant cell walls, as it primarily targets chitin, which is found in fungal cell walls and not in plant cells.

5. **Conclusion**:
- Based on the above information, the enzymes required to obtain protoplasts from plant cells are cellulase and pectinase. Therefore, the correct answer is both A (cellulase) and C (pectinase).

### Final Answer:
The enzymes required to obtain protoplast from a plant cell are **both A (cellulase) and C (pectinase)**.

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