You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful ?

Options

A. Isolate the genomic DNA from the organism, digest with a restriction endonuclease, Insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark
B. Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark
C. Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteria. Screen colonies for the ability to glow in the dark
D. Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark.

Explanation

To clone the gene responsible for the glowing trait in the novel eukaryotic organism, follow these steps:

### Step-by-Step Solution:

1. **Isolate mRNA from the organism**:
- Start by extracting the total RNA from the cells of the glowing organism. Since the glowing trait is likely due to a specific gene, you will need to isolate the mRNA that corresponds to this gene.

2. **Reverse Transcribe mRNA to cDNA**:
- Use reverse transcriptase to convert the isolated mRNA into complementary DNA (cDNA). This process synthesizes cDNA from the mRNA template, which represents the coding sequence of the gene without introns.

3. **Insert cDNA into a Plasmid Vector**:
- Once you have the cDNA, you need to insert it into a plasmid vector. This vector will carry the cDNA into a host cell. Ensure that the plasmid has the necessary elements for replication and expression in eukaryotic cells.

4. **Transform Eukaryotic Cells (e.g., Yeast)**:
- Introduce the plasmid containing the cDNA into a eukaryotic host, such as yeast. This transformation allows the host cell to take up the plasmid and express the glowing gene.

5. **Screen Colonies for the Ability to Glow**:
- After transformation, culture the yeast cells and screen the colonies for the ability to glow in the dark. This step helps identify which transformed cells successfully express the glowing trait.

### Final Answer:
The most successful strategy to clone the glowing gene is to isolate mRNA from the organism, reverse transcribe it to generate cDNA, insert the cDNA into a plasmid vector, and transform this into a eukaryotic cell such as yeast, followed by screening for glowing colonies.

---