What would be the base sequence of RNA transcript obtained from the given DNA segment ?
5' - G C A T T C G G C T A G T A A C-3` Coding strand of DNA
3' - C G T A A G C C G A T C A T C A T T G-5' Non - coding strand of DNA

Options

A. 5'-G C A U U C G G C U A G U A A C-3'
B. 5'- C G U A A G C C G A U C A U U G -3'
C. 5'- G C A T T C G G C T A G T A A C -3'
D. 3'- C G T A A G C C G A T C A T T G-5'

Explanation

To determine the base sequence of the RNA transcript obtained from the given DNA segment, we can follow these steps:

### Step 1: Identify the Coding Strand
The coding strand of DNA is given as:
5' - G C A T T C G G C T A G T A A C - 3'

### Step 2: Understand the Transcription Process
During transcription, the RNA polymerase synthesizes RNA from the DNA template strand. The RNA transcript is complementary to the non-coding strand and has the same sequence as the coding strand, except that thymine (T) in DNA is replaced by uracil (U) in RNA.

### Step 3: Write the RNA Sequence
Since the RNA transcript will have the same sequence as the coding strand but with T replaced by U, we can directly convert the coding strand into RNA:
- Replace each T with U.

The coding strand is:
5' - G C A T T C G G C T A G T A A C - 3'

Now, replacing T with U:
5' - G C A U U C G G C U A G U A A C - 3'

### Step 4: Final RNA Sequence
Thus, the base sequence of the RNA transcript is:
5' - G C A U U C G G C U A G U A A C - 3'

### Conclusion
The RNA transcript obtained from the given DNA segment is:
5' - G C A U U C G G C U A G U A A C - 3'

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