RFLP analysis is a technique that

Options

A. uses hybridization to detect specific DNA restriction fragments in genomic DNA
B. measures the transfer frequency of genes during conjugation
C. is used to detect genetic variations at protein loci
D. is used to amplify genes for producing useful products

Answer

### Step-by-Step Solution for RFLP Analysis

1. **Understanding RFLP**: RFLP stands for Restriction Fragment Length Polymorphism. It is a technique used to analyze the variations in DNA sequences among individuals.

2. **DNA Isolation**: The first step in RFLP analysis is to isolate the DNA from the cells of the individuals being studied. This is crucial because the quality and quantity of DNA will affect the results.

3. **PCR Amplification**: Once the DNA is isolated, it is amplified using the Polymerase Chain Reaction (PCR). This process increases the number of copies of the DNA, making it easier to analyze.

4. **Restriction Enzyme Treatment**: The amplified DNA is then treated with restriction enzymes. These enzymes cut the DNA at specific sequences, resulting in fragments of varying lengths.

5. **Gel Electrophoresis**: The resulting DNA fragments are separated using gel electrophoresis. This technique allows the fragments to be sorted based on their size, with smaller fragments moving faster through the gel than larger ones.

6. **Comparison of Fragments**: After separation, the lengths and types of the DNA fragments are compared. This comparison reveals differences in the DNA sequences among the individuals, which can be used to identify genetic variations.

7. **Conclusion**: The correct answer to the question is that RFLP analysis is a technique that uses hybridization to detect specific DNA fragment lengths in genomic DNA.