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Given below are two restriction endonucl...

Given below are two restriction endonuclease enzymes Hind II and Sal I. Write the name of genus, species and strain of the bacteria from which these have been obtained.

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**Step-by-Step Solution:** 1. **Identify the first restriction endonuclease: Hind II.** - Hind II is derived from a specific bacterium. - The genus is **Haemophilus**. - The species is **influenzae**. - The strain is denoted by the letter "d" which indicates the specific strain used for isolation. ...
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How many restriction endonuclease enzymes have been isolated till date from various bacteria?

Assertion: The name of any restriction endonuclease ends with a Roman number Reason :Roman number indicate the order in which the enzyme were discovered from that strain of bacteria

Knowledge Check

  • Read the following statements and select the correct ones. (i) Same kind of sticky ends are produced when a DNA has been cut by different restriction enzymes. (ii) Exonucleases make cuts at specific positions within the DNA. (iii) Hind ll was the first restriction endonuclease to be isolated. (iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA. (v) Presence of more than one recognition sites for a enzyme within the vector facilitates the gene cloning.

    A
    (i),(iii) and (v)
    B
    (ii) and (iv)
    C
    (iii) and (iv)
    D
    (ii),(iii) and (iv)

  • Which of the following statements are correct ? (i) Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site, but between the same two bases on the opposite strands. (ii) Hind ll always cuts DNA molecules at a particular point by reconginisng a specific sequence of six base pairs. (iii) Separated DNA fragments cannot be visualised without staining on an agarose gel electrophoresis. (iv) 'Ori' is the sequence responsible for controlling the copy number. (v) DNA is a positively charged molecule.

    A
    (i), (iii) and (v)
    B
    (i), (ii),(iii) and (iv)
    C
    (iii),(iv) and (v)
    D
    (i),(ii),(iii),(iv) and (v)

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