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For producing a recombinant protein (for...

For producing a recombinant protein (for therapeutic purpose) in large scale, which vector would you choose - a low copy number or high copy number?

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To determine the appropriate vector for producing a recombinant protein for therapeutic purposes on a large scale, we need to consider the copy number of the vector. ### Step-by-Step Solution: 1. **Understanding Vector Copy Number**: - The vector copy number refers to the number of copies of a plasmid present in a bacterial cell. This is crucial because it influences the amount of recombinant protein produced. 2. **Relationship Between Copy Number and Protein Production**: ...
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Stabilities of alkanes can be compared by converting these compounds to a common product and comparing the amount of the heat given off. One possibility would be to measure the heat of combustion from converting alkenes to CO_(2) and H_(2)O . The heats of combustion are of large values and measuring small difference in these large numbers is difficult. Alkene of the lowest heat of combustion among isomeric alkenes is of the lowest energy and is most stable. Th stability of alkenes is often compared by meansuring the ehat of hydrogenation 9 heat given off, Delta H_(h)^(@) during catalytic hydrogenation. The heat of hydrogenation is in smal number, which provides more accurate energy difference. For a compound containing more than one double bond, Delta_(h)^(@) is the sum of heat of hydrogenation of individual double bonds. For non - conjugated diens, this additive relatioship is found to hold. For conjugated dienes, however, the measured value is slightly lower than expected. Cumulated dienes, which are even less stable than non - conjugated dienes. The more stable is the alkene, lower is the heat of combustion and heat of hydrogenation. More highly substituted double bonds are usually more stable. In case of cyclokanes, compounds having higher angle strin are less stable. In which of the following is Delta H^_(h)^(@) the maximum ?

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