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What is gene cloning?...

What is gene cloning?

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Gene cloning refers to the process by which multiple copies of gene of interest are obtained by recombinant DNA technology.
It involves following steps: (a) Isolation of gene of interest. (b) Ligation of gene of interest (DNA segment) to a vector plasmid (Formation of recombinant DNA).
(c) Introduction of recombinant DNA into a bacterial ceU (Transformation of host cell).
(d) Multiplication of transformed host bacterial cell to have colony of transformed cells.
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