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A plasmid DNA and a linear DNA (both are...

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain

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### Step-by-Step Solution: 1. **Understanding the Structure of DNA**: - Plasmid DNA is a circular DNA molecule, while linear DNA is a straight, linear structure. This fundamental difference in structure is crucial for understanding how they behave when cut by restriction endonucleases. 2. **Cutting with Restriction Endonuclease**: - Both the plasmid DNA and the linear DNA have one site for a restriction endonuclease. When the restriction enzyme cuts the plasmid DNA, it opens the circular structure, resulting in one linear fragment of DNA. - In contrast, when the linear DNA is cut at its restriction site, it will split into two separate linear fragments. ...
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