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In an experiment of recombinant DNA tech...

In an experiment of recombinant DNA technology, in a bacterial culture some of the colonies produced blue colour in presence of a chromogenic substrate. Why?

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**Step-by-Step Solution:** 1. **Understanding Recombinant DNA Technology**: In recombinant DNA technology, DNA from different sources is combined to create new genetic combinations. This often involves inserting a gene of interest into a plasmid vector. 2. **Use of Chromogenic Substrate**: In many experiments, a chromogenic substrate is used to visualize the activity of certain enzymes. One common substrate is X-gal, which is used to detect the activity of the enzyme beta-galactosidase. 3. **Differentiating Between Recombinants and Non-Recombinants**: When the plasmid vector is used to introduce a gene into bacterial cells, it can either be successful (recombinant) or unsuccessful (non-recombinant). The successful insertion of the gene can disrupt the function of existing genes in the plasmid. ...
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