What is the use of Southern blotting technique?

Explanation

### Step-by-Step Solution:

1. **Definition of Southern Blotting**: Southern blotting is a molecular biology technique used to detect specific DNA sequences within a complex mixture of DNA.

2. **DNA Fragmentation**: The first step in the Southern blotting technique involves using restriction enzymes to cut the DNA into smaller fragments. These enzymes recognize specific sequences in the DNA and cleave them, resulting in a collection of DNA fragments.

3. **Gel Electrophoresis**: After the DNA has been fragmented, the next step is to separate these fragments based on their size using gel electrophoresis. In this process, the DNA fragments are loaded into a gel matrix and an electric current is applied. The negatively charged DNA moves towards the positive electrode, allowing smaller fragments to migrate faster than larger ones.

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