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What is the use of alkaline phosphatase?...

What is the use of alkaline phosphatase?

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**Step-by-Step Solution:** 1. **Understanding the Role of Alkaline Phosphatase**: Alkaline phosphatase is an enzyme that plays a crucial role in molecular biology, particularly in the context of DNA manipulation. 2. **DNA Fragment Preparation**: In biotechnology, DNA fragments are often cut at specific sites using restriction endonucleases. These enzymes recognize specific sequences in the DNA and cleave it, resulting in fragments that can be used for cloning or other applications. 3. **Self-Ligation Issue**: After DNA fragments are cut, there is a possibility of self-ligation, where the ends of the same DNA fragment can join together without the need for a vector or another DNA fragment. This can lead to unwanted products in cloning experiments. ...
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