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How is DNA made visible in gel electroph...

How is DNA made visible in gel electrophoresis?

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### Step-by-Step Solution: 1. **Preparation of Gel**: The first step in gel electrophoresis is to prepare the agarose gel, which acts as a medium for the separation of DNA fragments. 2. **Loading DNA Samples**: Once the gel is prepared and solidified, DNA samples mixed with a loading dye are loaded into the wells of the gel. 3. **Running the Gel**: An electric current is applied across the gel, causing the negatively charged DNA fragments to migrate towards the positive electrode. Smaller fragments move faster than larger ones, resulting in separation based on size. ...
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In electrophoresis :-

Which of the following is correct regarding the separation of DNA fragments during gel electrophoresis?

Which of the following compound is used in visualisation of DNA fragments in gel electrophoresis?

In agarose gel electrophoresis–

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below

a) How do DNA fragments migrate and resolve in a Gel electrophoresis? b) How lane one is different from lane 2,3 and 4 in the GEL electrophoresis set up? c) How pure DNA fragments are made observable in the visible light?

Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes.

How can DNA segment, separated by gel electrophoresis be visualised and isolated ?