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Restriction endonuclease...

Restriction endonuclease

A

Cuts the DNA molecule randomly

B

Cuts the DNA molecule at specific sites

C

Restricts the synthesis of DNA inside the nucleus

D

Synthesises DNA

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### Step-by-Step Solution: 1. **Understanding Restriction Endonucleases**: - Restriction endonucleases, also known as restriction enzymes, are proteins that cut DNA at specific sequences. They are crucial tools in molecular biology and biotechnology. 2. **Function of Restriction Endonucleases**: - These enzymes act as molecular scissors, recognizing specific sequences in the DNA known as restriction sites. 3. **Specificity of Cuts**: - Unlike random cutting, restriction endonucleases cut DNA at precise locations. This specificity allows for targeted manipulation of DNA, which is essential in genetic engineering. 4. **Example of Restriction Sites**: - A common example of a restriction site is the sequence "AAGGCC". The enzyme will recognize and cut the DNA at this specific sequence. 5. **Clarifying Misconceptions**: - It is important to note that restriction endonucleases do not restrict the synthesis of DNA inside the nucleus, nor do they synthesize DNA. Their primary function is to cut DNA at specific sites. 6. **Conclusion**: - The correct answer to the question about the function of restriction endonucleases is that they cut DNA at specific sites.
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Read the given statements and select the correct option, Statement 1 : Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA Statement 2: Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

Assertion (A) : Restriction endonucleases recognise short pallindromic sequence and cut at specific sites Reason (R) : When a restriction endonuclease acts on a pallindrome, it cleaves both the strands of DNA molecule.

Select the correct option regarding restriction endonuclease P. 900 restriction enzyme have been isolated from over 200 strains. Q. The first restriction exonuclease was Hind II. R. Each restriction endonuclease recognises same palindromic sequence .

ICSE-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-COMPETITION CORNER (OBJECTIVE TYPE QUESTIONS)
  1. Which of the following is used in recombinant DNA technique ?

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  2. Which of the following is not a restriction endonuclease?

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  3. Restriction endonuclease

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  4. Molecular scissors, which cut DNA at specific site is

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  5. Genetically engineered bacteria are being employed for production of

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  6. Which one is regarded as a molecular scissors in biotechnology?

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  7. An institution where valuable plant material is likely to become irret...

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  8. Genetic engineering has been successfully used for producing

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  9. Restriction endonucleases are enzymes which

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  10. DNA or RNA segment tagged with a radioactive molecule is called

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  11. Which one of the following palindromic base sequences in DNA can be ea...

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  12. The vector for T-DNA is

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  13. Which of the following is a plasmid?

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  14. The mobile genetic element is

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  15. In recombinant DNA technique, the term vector refers to

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  16. Enzyme that is used in PCR technology is

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  17. GAATTC is the recognition site for the resetriction endonuclease

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  18. Satellite DNA is useful tool in ;

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  19. Which one of the following is used as vector for cloning genes into hi...

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  20. The technique of DNA fingerprinting was initially developed by

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