Electroporation procedure involves:

Options

A. Fast passage of food through sieve pores in phloem elements with the help of electric stimulation
B. Opening of stomatal pores during night by artificial light
C. Making transient pores in the cell membrane to introduce gene constructs
D. Purification of saline water with the help of a membrane system

Answer

### Step-by-Step Solution:

1. **Understanding Electroporation**:
Electroporation is a technique used to introduce substances such as DNA into cells by applying an electric field. This process is particularly relevant in the context of plant biotechnology.

2. **Mechanism of Electroporation**:
In electroporation, an electric field is applied to the cells, which causes temporary openings (or pores) to form in the cell membrane. This is due to the reconstruction of the bilipid layer of the membrane.

3. **Formation of Pores**:
The electric field induces the formation of hydrophilic pores in the bilipid layer of the cell membrane. These pores allow for the passage of molecules, such as gene constructs, into the cell.

4. **Purpose of Electroporation**:
The primary goal of electroporation is to facilitate the introduction of gene constructs into the plant's protoplast. This transformation is essential for genetic engineering and plant breeding.

5. **Identifying the Correct Option**:
Among the provided options, the correct one is:
- **Third Option**: Making transient pores in the cell membrane to introduce the gene constructs. This accurately describes the electroporation process.

### Final Answer:
The electroporation procedure involves making transient pores in the cell membrane to introduce the gene constructs.

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