Somatic hybridisation is accomplished by

Options

A. Grafting between two varieties
B. Fusion of two protoplast of different varieties of plants
C. Chromosome doubling in androgenic culture
D. Recombinant DNA technology

Answer

**Step-by-Step Solution:**

1. **Understanding Somatic Hybridization**: Somatic hybridization is a technique used to fuse two different plant cells or organisms without involving sexual reproduction. This process allows for the combination of genetic material from two different varieties.

2. **Protoplast Fusion**: The key method of somatic hybridization is through the fusion of protoplasts. Protoplasts are plant cells that have had their cell walls removed. The fusion occurs when the protoplasts of two different plant varieties come into close proximity and merge to form a hybrid cell.

3. **Evaluating the Options**:
- **Option 1: Grafting between two varieties**: This is incorrect. Grafting involves physically joining parts of two plants, which is not the same as somatic hybridization.
- **Option 2: Fusion of the two protoplasts of different varieties of the plant**: This is correct. This describes the process of somatic hybridization accurately.
- **Option 3: Chromosome doubling and androgenic culture**: This is incorrect. Chromosome doubling is a different process and not directly related to somatic hybridization.
- **Option 4: Recombination DNA technology**: This is also incorrect. Recombination DNA technology involves manipulating DNA and is not the same as somatic hybridization.

4. **Conclusion**: The correct answer to the question "Somatic hybridization is accomplished by" is **Option 2: Fusion of the two protoplasts of different varieties of the plant**.

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