Protoplasts of two different cells can be made to fuse with the help of

Options

A. Polyethylene glycol (PEG)
B. Calcium chloride
C. Sodium bicarbonate
D. HCl

Answer

To answer the question of how protoplasts of two different cells can be made to fuse, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Protoplasts**:
- Protoplasts are the living parts of plant cells, which include the cytoplasm, nucleus, and organelles, but lack the cell wall.

2. **Methods of Fusion**:
- There are various methods to induce the fusion of protoplasts. The most common methods include:
- **Electrofusion**: This technique uses an electric field to make the cell walls porous, allowing the protoplasts to fuse.
- **Chemical Fusion**: Certain chemicals can facilitate the fusion of protoplasts.

3. **Identifying the Correct Chemical**:
- Among the options provided (polyethylene glycol, calcium chloride, sodium bicarbonate, and HCl), polyethylene glycol (PEG) is known to be effective in promoting the fusion of protoplasts when used in conjunction with calcium ions.

4. **Conclusion**:
- Therefore, the correct answer to the question is that protoplasts of two different cells can be fused with the help of **polyethylene glycol (PEG)**.

### Final Answer:
Protoplasts of two different cells can be made to fuse with the help of **polyethylene glycol (PEG)**.

---