Identify the plasmid among following

Options

A. Hind III
B. Pbr322
C. `lambda`-phage
D. Both (2) & (3)

Answer

To identify the plasmid among the given options, we will analyze each option step by step.

### Step-by-Step Solution:

1. **Understanding Plasmids**:
- Plasmids are small, circular, double-stranded DNA molecules that are distinct from chromosomal DNA. They are typically found in bacteria and can replicate independently. Plasmids often carry genes that provide bacteria with genetic advantages, such as antibiotic resistance.

2. **Analyzing Option 1: HIND 3**:
- HIND 3 is not a plasmid. It is a restriction endonuclease enzyme derived from the bacterium Haemophilus influenzae. Its function is to cut DNA at specific sites, known as restriction sites. Therefore, this option is incorrect.

3. **Analyzing Option 2: PBR 322**:
- PBR 322 is a well-known plasmid vector used in genetic engineering. It is 4361 base pairs in size and contains an origin of replication (ORI) which allows it to replicate within a host. It also has multiple cloning sites (MCS) and antibiotic resistance genes (ampicillin and tetracycline). Since it meets the criteria of a plasmid, this option is correct.

4. **Analyzing Option 3: Lambda**:
- Lambda (λ) refers to a type of bacteriophage, which is a virus that infects bacteria. While it can be used as a vector for genetic engineering, it is not a plasmid. Therefore, this option is incorrect.

5. **Analyzing Option 4: Both 2 and 3**:
- Since option 3 (Lambda) is not a plasmid, this option is also incorrect.

### Conclusion:
The correct answer is **PBR 322**, which is a plasmid.

### Final Answer:
**PBR 322 is the plasmid among the given options.**