Boliver and Rodriguez developed

Options

A. Shuttle vector
B. pBR322
C. Puc19
D. Both (1) & (2)

Answer

### Step-by-Step Solution:

1. **Identify the Scientists and Their Contribution**:
Bolivar and Rodriguez are the scientists credited with developing a specific plasmid vector.

2. **Determine the Name of the Vector**:
The vector developed by Bolivar and Rodriguez is known as PBR 322.

3. **Understand the Characteristics of PBR 322**:
- PBR 322 is a plasmid vector that is approximately 4361 base pairs in length.
- It contains an Origin of Replication (ORI), which is essential for the vector to replicate within a host cell.
- It has two selectable markers for antibiotic resistance: one for ampicillin and another for tetracycline.

4. **Clarify the Function of PBR 322**:
PBR 322 is primarily used as a cloning vector, which means it is utilized to clone DNA fragments.

5. **Evaluate the Options Given**:
- **Shuttle Vector**: This is incorrect because a shuttle vector can function in both prokaryotic and eukaryotic cells, which PBR 322 does not.
- **PBR 322**: This is correct as it is the vector developed by Bolivar and Rodriguez.
- **PUC 19**: This is incorrect because it was not developed by these scientists.
- **Both 1 and 2**: This is also incorrect since only PBR 322 is correct.

6. **Select the Correct Answer**:
The correct answer is **PBR 322**.