Isolation of genetic material from fungal cells involves the use of

Options

A. Lysozyme
B. Cellulase
C. Chitinase
D. Polymerase

Answer

To isolate genetic material from fungal cells, the following steps are typically involved:

### Step-by-Step Solution:

1. **Understanding the Fungal Cell Structure**:
- Fungal cells have a rigid cell wall primarily composed of chitin. This structure needs to be disrupted to access the genetic material inside.

2. **Choosing the Appropriate Enzyme**:
- To break down the chitin in the fungal cell wall, the enzyme chitinase is used. Chitinase specifically targets and degrades chitin, allowing for the release of the cellular contents.

3. **Cell Lysis**:
- The addition of chitinase to the fungal cell suspension will lead to the degradation of the cell wall. This process is known as cell lysis, which releases the internal components, including the DNA.

4. **Purification of DNA**:
- After lysis, the mixture may contain cellular debris along with the DNA. To purify the DNA, further steps such as centrifugation or filtration may be employed to separate the DNA from other cellular components.

5. **Precipitation of DNA**:
- The DNA can be precipitated out of the solution using alcohol (ethanol or isopropanol). This step helps in collecting the DNA in a solid form.

6. **Washing and Resuspension**:
- The precipitated DNA is washed with alcohol to remove impurities and then resuspended in a suitable buffer for further analysis or storage.

### Final Answer:
The isolation of genetic material from fungal cells involves the use of **chitinase**.

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