Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is called

Options

A. Gene Gun method
B. Biolistic method
C. Microinjection method
D. Direct DNA injection method

Answer

To solve the question regarding the method in which recombinant DNA is directly injected into the nucleus of an animal cell using microneedles, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Context**: The question asks for a specific method of introducing recombinant DNA into animal cells. It's important to note that this process involves the direct injection of DNA into the cell's nucleus.

2. **Identifying the Method**: The method that involves injecting DNA directly into the nucleus of an animal cell using microneedles is known as **microinjection**. This technique is commonly used in genetic engineering to introduce foreign DNA into cells.

3. **Explaining Microinjection**: In microinjection, a fine glass needle (microneedle) is used to inject the desired DNA directly into the nucleus of the target cell. This method is particularly effective for oocytes (egg cells) and other animal cells.

4. **Distinguishing from Other Methods**: While there are other methods for introducing DNA into cells, such as the gene gun method (biolistic method), these are primarily used for plant cells, not animal cells. Therefore, they are not applicable in this context.

5. **Conclusion**: Based on the above steps, the correct answer to the question is that the method of injecting recombinant DNA into the nucleus of an animal cell using microneedles is called **microinjection**.

### Final Answer:
**Microinjection**