Plasmids

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Discuss with your teacher and find out how to distinguish between (a) Plasmid DNA and Chromosomal DNA (b) RNA and DNA (c) Exonuclease and Endonuclease

Match each term on the right with the definition on the left, Column - I A) Bacterial enzymes used to cut DNA at defined sequences B) Sequences cut by restriction enzymes C) Ends left on DNA segments cut by DNA restriction enzymes D) Circular pieces of DNA found in bacteria E) Bacterial viruses F) Process by which bacteria take up pieces of DNA from the environment G) Plasmid DNA that has incorporated human DNA H) Term used to describe any vehicle that moves DNA from the organism to another Column - II I) Transformation II) Cloning vector III) Recombinant IV) Recognition sequences V) Plasmids VI) Sticky ends VII) Restriction enzymes VIII) Bacteriophages

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

What is a plasmid ? What is its significance ?

Ori sequence in plasmid refers to sequence

In recombinant DNA technology, a plasmid vector must be cleaved by

The most important feature in a plasmid to be used as a vector is

Transgenic plants are produced by using Ti-plasmid from