Enzyme required for polymerase chain reaction (PCR) is

Options

A. RNA polymerase
B. Ribonuclease
C. Taq polymerase
D. Endonuclease

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the enzyme required for the polymerase chain reaction (PCR). PCR is a technique used to amplify DNA.

2. **Analyzing the Options**: The options provided are:
- RNA polymerase
- Ribonuclease
- Taq polymerase
- Endonuclease

3. **Evaluating Each Option**:
- **RNA Polymerase**: This enzyme is responsible for synthesizing RNA from a DNA template during transcription. It is not involved in PCR, so this option is incorrect.
- **Ribonuclease**: This enzyme degrades RNA into smaller components. It does not play a role in DNA amplification, making this option incorrect as well.
- **Taq Polymerase**: This is a type of DNA polymerase that is thermostable, meaning it can withstand the high temperatures used in PCR. Its primary function in PCR is to amplify DNA, making it the correct choice.
- **Endonuclease**: This enzyme is involved in DNA repair and cuts DNA at specific sites. It is not used in PCR, so this option is also incorrect.

4. **Conclusion**: The enzyme required for the polymerase chain reaction (PCR) is Taq polymerase.

**Final Answer**: The enzyme required for polymerase chain reaction (PCR) is Taq polymerase.

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