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E. coli cloning vector pBR322 contains r...

E. coli cloning vector pBR322 contains restriction sites in the region of `amp^( R)`, `tet^(R )` genes that codes for the

A

selection of recombinants from non-recombinants

B

antibiotic resistance gene

C

foreign DNA

D

origin

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The correct Answer is:
B
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Explore conceptually related problems

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

P^(BR322) cloning vector has the genes for resistance against

Unique restriction sites are present in a small region of DNA in a vector . What is this region called /

The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322.

(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play. (b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

Which statement is suitable for tet^(R) gene

VMC MODULES ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-IMPECCABLE
  1. E. coli cloning vector pBR322 contains restriction sites in the region...

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  2. Plasmids are suitable vectors for gene cloning because :

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  3. What are true of Plasmids

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  4. The linking of antibiotic resistance gene with the plasmid vector beca...

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  5. Main objective of production/use of herbicide resistant GM crops is to...

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  6. Thermal cycler is used in the reaction:

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  7. Which of the following cut DNA at specific sites ?

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  8. The autonomously independent self replicating extranuclear DNA imparti...

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  9. Polyethylene glycol method is used for

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  10. Which one of the following is commonly used in transfer of foreign DNA...

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  11. Ti-plasmids used in genetic engineering is obtained from :

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  12. Electroporation procedure involves:

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  13. Palaeontologists unearthed a human skull during excavation. A small fr...

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  14. Which one of the following palindromic base sequences in DNA can be e...

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  15. Which of the following DNA sequence qualifies to be designted as a pal...

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  16. Enzymes that cleave nucleic acids within the polynucleotide chain is k...

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  17. A mixture containing DNA fragments A,B,C and D, with molecular weights...

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  18. Which of the following techniques is used to separate proteins?

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  19. Which one of the following is used as vector for cloning genes into hi...

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  20. Which of the following are used in gene cloning ?

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  21. In recombinant DNA technique, the term vector refers to :

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