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After electrophoresis the separated DNA ...

After electrophoresis the separated DNA fragment can be visualized ethidium bromide gel exposed UV light . These DNA fragments appear as _______ coloured bands :

A

cloning

B

Transformation

C

PCR

D

Clonal selection

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The correct Answer is:
To answer the question regarding the appearance of DNA fragments after electrophoresis when visualized with ethidium bromide under UV light, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding Electrophoresis**: Electrophoresis is a technique used to separate DNA fragments based on their size. DNA fragments are negatively charged, and when an electric field is applied, they migrate towards the positively charged electrode. 2. **Separation of DNA Fragments**: The smaller DNA fragments move faster and travel further through the gel compared to larger fragments. This results in a separation of DNA fragments according to their size. 3. **Staining with Ethidium Bromide**: After the electrophoresis process, the separated DNA fragments are stained with a compound called ethidium bromide. Ethidium bromide intercalates between the bases of the DNA. 4. **Visualization Under UV Light**: When exposed to ultraviolet (UV) light, the ethidium bromide emits fluorescence, allowing the DNA fragments to be visualized as bands on the gel. 5. **Appearance of DNA Bands**: The DNA fragments appear as bright orange or pink colored bands when viewed under UV light due to the fluorescence emitted by the ethidium bromide. 6. **Conclusion**: Therefore, the correct answer to the question is that the DNA fragments appear as **orange or pink colored bands**. ### Final Answer: The DNA fragments appear as **orange or pink colored bands**.
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