Isolation of the genetic material in pure form, free from other macromolecules can be achieved by treating the bacterial cells/plant or animal tissues with the following enzymes, except

Options

A. lysozyme
B. cellulase
C. chitinase
D. ligase

Answer

To solve the question regarding the isolation of genetic material in pure form from bacterial cells, plant tissues, or animal tissues using specific enzymes, we need to analyze the roles of the mentioned enzymes and identify the one that does not belong to the group used for this purpose.

### Step-by-Step Solution:

1. **Understanding DNA Isolation**:
- DNA isolation is the process of extracting DNA from cells while ensuring it is free from other macromolecules such as proteins, RNA, and lipids. This is crucial for genetic engineering and molecular biology applications.

2. **Role of Enzymes in Cell Lysis**:
- To isolate DNA, the cell membrane and cell wall (if present) must be disrupted to release the DNA. This is typically achieved using specific enzymes that target the components of the cell wall.

3. **Identifying the Enzymes**:
- **Lysozyme**: This enzyme is effective against bacterial cell walls, breaking down peptidoglycan, which is essential for releasing DNA from bacterial cells.
- **Cellulase**: This enzyme targets cellulose, which is a major component of plant cell walls. It helps in breaking down the cell wall to release DNA from plant tissues.
- **Chitinase**: This enzyme acts on chitin, which is found in the cell walls of fungi. It is used for isolating DNA from fungal cells.

4. **Analyzing the Options**:
- The enzymes listed (lysozyme, cellulase, chitinase) are all involved in breaking down cell walls of their respective organisms (bacteria, plants, and fungi).
- **Ligase**: This enzyme is not involved in the breakdown of cell walls. Instead, ligase is used in DNA replication and repair processes to join DNA fragments together.

5. **Conclusion**:
- The enzyme that does not belong to the group of enzymes used for the isolation of genetic material is **ligase**.

### Final Answer:
The correct answer is **ligase**.

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