Mark an incorrect statement :

Options

A. In microinjection technique of gene transfer only one cell can be injected at a time
B. PCR can detect a mutation able to cause a particular disease
C. In denaturation step of PCR the target DNA is heated to `42^(@)`C to `46^(@)` C only
D. The use of restriction enzymes is proceeded by use of chitinases, pectinases, lysozyme etc.

Answer

To solve the question, we need to evaluate each of the provided statements about biotechnology techniques and identify which one is incorrect.

### Step-by-Step Solution:

1. **Analyze Option A**:
- Statement: "In microinjection technique of gene transfer, only one cell can be injected at a time."
- Evaluation: This statement is correct. The microinjection technique involves injecting DNA into a single cell using a fine needle.

2. **Analyze Option B**:
- Statement: "PCR can detect a mutation able to the cause of particular disease."
- Evaluation: This statement is also correct. Polymerase Chain Reaction (PCR) is used to amplify DNA and can be used to detect mutations associated with diseases.

3. **Analyze Option C**:
- Statement: "In the denaturation step of the PCR, the target DNA is heated to 42 to 46 degrees Celsius."
- Evaluation: This statement is incorrect. During the denaturation step of PCR, the DNA is heated to approximately 90 degrees Celsius to separate the strands, not 42 to 46 degrees Celsius.

4. **Analyze Option D**:
- Statement: "The use of restriction enzyme is preceded by the use of chitinase, pectinase, and lysozyme."
- Evaluation: This statement is correct. These enzymes are used to break down the cell wall components before restriction enzymes can act on the DNA.

### Conclusion:
The incorrect statement is **Option C**: "In the denaturation step of the PCR, the target DNA is heated to 42 to 46 degrees Celsius."

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