First artificial recombinant DNA molecule was created by:

Options

A. linking of gene encoding antibiotic resistance with a native plasmid of Salmonella typhimuriumn
B. linking of gene encoding fertility factor with a na- tive plasmid o E. coli
C. linking of gene encoding .-galactosidase with a native plasmid of Agrobacterium tumefac iens
D. linking of gene encoding lactase with a native plas-mid off. coli

Answer

To determine who created the first artificial recombinant DNA molecule, we can follow these steps:

1. **Understanding Recombinant DNA**: Recombinant DNA refers to DNA that has been artificially formed by combining constituents from different organisms. This technology allows for the manipulation of genetic material.

2. **Identifying Key Contributors**: The question asks about the first artificial recombinant DNA molecule. This was created by researchers in the early 1970s.

3. **Analyzing the Options**:
- **Option 1**: Linking of gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium.
- **Option 2**: Linking of gene encoding fertility factor with a native plasmid of E. coli.
- **Option 3**: Linking of gene encoding Galactosidase with a native plasmid of Agrobacterium tumefaciens.
- **Option 4**: Linking of gene encoding Lactase with a native plasmid equivalent.

4. **Research Findings**: The first successful creation of recombinant DNA was achieved by Paul Berg and his team, where they linked an antibiotic resistance gene with a plasmid from Salmonella typhimurium in 1972.

5. **Conclusion**: Based on the analysis, the correct answer is **Option 1**: linking of gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium.