Virus free plants from a virus infected plant can be obtained through:

Options

A. Root tip culture
B. Shoot tip culture
C. Callus culture
D. Suspension culture

Answer

To obtain virus-free plants from a virus-infected plant, the most effective method is through meristem culture. Here’s a step-by-step solution:

### Step 1: Understanding Virus Infection in Plants
- **Explanation**: When a plant is infected by a virus, the virus is distributed unevenly throughout the plant. Certain areas, particularly the meristematic tissues, are less likely to contain the virus.
- **Hint**: Think about where in a plant the virus is least concentrated.

### Step 2: Identifying Meristematic Tissue
- **Explanation**: Meristematic tissues are regions in plants where cells are actively dividing. These tissues are found at the tips of roots and shoots. Since meristematic tissues are actively growing, they are less likely to harbor viruses compared to other parts of the plant.
- **Hint**: Recall the function of meristems in plant growth.

### Step 3: Utilizing Tissue Culture Techniques
- **Explanation**: Tissue culture is a technique used to grow new plants from small sections of the plant, such as meristems. By isolating and culturing the meristematic tissue, we can produce new plants that are free from the virus.
- **Hint**: Consider how tissue culture can help propagate plants.

### Step 4: Evaluating the Given Options
- **Explanation**: The options provided include:
1. Root tip culture
2. Shoot tip culture
3. Callus culture
4. Suspension culture

- **Root tip culture**: This method focuses on root development and does not produce new plants.
- **Shoot tip culture**: This method involves culturing the shoot tip, which contains meristematic tissue, making it a viable option for obtaining virus-free plants.
- **Callus culture**: This involves disorganized plant cells and does not ensure virus-free plants.
- **Suspension culture**: This method grows cells in a liquid medium and does not lead to the production of whole plants.
- **Hint**: Analyze each option based on whether it involves meristematic tissue and the potential to produce new plants.

### Step 5: Conclusion
- **Final Answer**: The correct method to obtain virus-free plants from a virus-infected plant is through **shoot tip culture** (Option 2), as it utilizes meristematic tissue where the virus is absent.