The pH of nutrient medium in plant tissue culture is adjusted between _________.

Options

A. `3 - 4`
B. `4.1 - 4.8`
C. `5 - 5.8`
D. `6 - 7`

Answer

To answer the question regarding the pH of the nutrient medium in plant tissue culture, we will follow these steps:

### Step-by-Step Solution:

1. **Understand the Question**: The question asks for the optimal pH range for the nutrient medium used in plant tissue culture.

2. **Identify the Options**: The options provided are:
- A) 3 to 4
- B) 4.1 to 4.8
- C) 5 to 5.8
- D) 6 to 7

3. **Analyze Each Option**:
- **Option A (3 to 4)**: This pH range is too low for plant tissue culture and is typically used before sterilization.
- **Option B (4.1 to 4.8)**: While this range is slightly better, it is still not optimal for cell division and development.
- **Option C (5 to 5.8)**: This is the recommended pH range for nutrient media as it supports proper cell division and mineral absorption.
- **Option D (6 to 7)**: This range is too high as it can cause the culture medium (like agar) to harden, which is not suitable for plant tissue culture.

4. **Select the Correct Answer**: Based on the analysis, the correct answer is **Option C (5 to 5.8)**, as it is the optimal pH range for plant tissue culture.

5. **Conclusion**: The pH of the nutrient medium in plant tissue culture is adjusted between **5 to 5.8**.